LMU Munich — Faculty of Biology — Department Human Biology and BioImaging
Epigenetics deals with molecular mechanisms that lead to the cell-specific reading of genetic information. They cause the majority of genes in somatic cells to be permanently downregulated or silenced during embryonic development and only a smaller fraction to be expressed.
Our group is particularly interested in the function and regulation of DNA methylation in mammalian cells. A major focus is on the complex interplay between the DNA methyltransferases involved (Dnmt1/Dnmt3a 3b) and the factors interacting with them.
We draw on a broad spectrum of analyses combining classical biochemical, molecular-genetic and cell biological methods. We are constantly expanding these with novel methods and approaches. Techniques developed or newly established in the group include nanobody/chromobody technology, DNA methyltransferase activity determination in vitro and in vivo, fluorescence-two/three-hybrid (F2H/F3H) assay and mathematical modeling of FRAP experiments to characterize protein interactions and dynamics in living cells, and super-resolution 3D microscopy with 3‑dimensional structured illumination (3D-SIM).